Mastering Q-PCR
- Overview
- Course Content
- Requirements & Materials
COURSE ID: BIOE 1050P
Contact for course-related questions
Gregory A. HairMastering Q-PCR
Course Description
This comprehensive workshop is tailor-made for individuals seeking to master the techniques essential for successful Q-PCR implementation and analysis. By the end of this course, participants will possess the skills to conceive, execute, and interpret Q-PCR experiments, design primers, and develop custom Q-PCR programs. Here's what this workshop has in store for you:
- Grasp the fundamental and advanced principles of Quantitative PCR.
- Acquire practical expertise in Q-PCR, including program development, primer design, and data analysis.
- Explore the diverse types of Q-PCR available and their applications.
- Recognize the challenges and limitations of Q-PCR, such as sensitivity, specificity, and cost considerations.
TOPIC Q-PCR
- Q-Pcr theory/background
- Quiz to Indicate Mastery
TOPIC Primer and Probe Design
- Basic Primer Design - Quiz to Indicated Mastery
- Basic Probe Design – Quiz to indicate Mastery.
TOPIC Primer and Probe Design & Detection
- Advanced Primer and Probe Design
- Probe Detection and Analysis
TOPIC Q-PCR setup & implementation
- Various Q-PCR Setup Protocols & Programming
- Implementation of Participant Practice Protocol
TOPIC Data Analysis, Vendor participation
- Analysis of Participant Q-PCR data
- Conlusions, Vendor Participation, End Luncheon.
Prerequisites
Undergraduates
Major in a chemical, physical, biological sciences and approval of advisor (determination of sufficient background knowledge, competence and commitment). Successful completion EHS mandated classes Laboratory Safety 101, Right to know and Bloodborne Pathogens.
Postgraduates
Approval of advisor or Primary Investigator (determination of sufficient background knowledge, competence, and commitment). Successful completion EHS mandated classes Laboratory Safety 101, Right to know and Bloodborne Pathogens.
Employees
Approval of Primary Investigator (determination of sufficient background knowledge, competence, and commitment). Successful completion EHS mandated classes Laboratory Safety 101, Right to know and Bloodborne Pathogens.
Faculty/Prof.
Interest and Commitment to complete 3 – 4 two-hour sessions. Successful completion EHS mandated classes Laboratory Safety 101, Right to know and Bloodborne Pathogens.
Materials
Required:
- Lab Notebooks, Lecture Notebooks
- Laptops, Wi-Fi connectivity, and traditional learning implements
Provided:
Lecture and Laboratory Instructional materials.
Access to all laboratory instrumentation and supplies.
Access to company representatives
Post-workshop consultation services at no charge.
Access to equipment and instrumentation
Who Should Attend
This workshop is ideal for those individuals seeking an enhanced understanding and practical implementation of Q-PCR. This may include:
- Working professionals or postgraduates looking to enhance their understanding and technical implementation of Q-PCR.
- Undergraduate or Graduate Students interested in learning Q-PCR theory and rigorous practical implementation.
- Individuals (Bioengineers, Project Managers, etc.) seeking a greater understanding of Q-PCR to help with experimental design and interpretation.
What You Will Learn
- Awareness of Q-PCR procedures currently available, Q-PCR sample preparation and primer design, applicability of various Q-PCR protocols to various experimental needs.
- Deeper understanding of Q-PCR theory and practical limitations.
- Insight into understanding the quantitation of purified DNA and QC of DNA preparations and developing the ability to interpret Q-PCR assays.
- Ability to critically assess scientific literature utilizing various Q-PCR methodologies.
How You Will Benefit
- Design and implement basic, multiplex and nested Q-PCR experiments.
- Analyze and interpret Q-PCR experimental data
- Gain a deeper and more relevant understanding of experimental and clinical applications of Q-PCR
- Perform QC on Q-PCR experiments successfully.
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Grow Your Professional Network
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Taught by Experts in the Field
The course schedule was well-structured with a mix of lectures, class discussions, and hands-on exercises led by knowledgeable and engaging instructors.
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GTPE does not have a discount program for senior citizens. However, Georgia Tech offers a 62 or Older Program for Georgia residents who are 62 or older and are interested in taking for credit courses. This program does not pay for noncredit professional education courses. Visit the Georgia Tech Undergraduate Admissions page for more information on the undergraduate program and the Georgia Tech Graduate Admissions page for more information on the graduate program.
GTPE does not have a program for senior citizens. However, Georgia Tech offers a 62 or Older Program for Georgia residents who are 62 or older and are interested in taking for credit courses. This program does not pay for noncredit professional education courses. Visit the Georgia Tech Undergraduate Admissions page for more information on the undergraduate program and the Georgia Tech Graduate Admissions page for more information on the graduate program.
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GTPE does not issue transcripts or certificates with Professional Develop Hours (PDH) or Professional Development Units (PDU), but the crosswalk here is provided for reference.
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